Dr. Hélène Munier-Lehmann (IP), Dr. Helen Boyd (AZ)
Identification and characterization of allosteric sites of UMP kinase
About the project:
My Ph.D. project focuses on the identification and characterization of allosteric sites of the uridine monophosphate kinase of Mycobacterium tuberculosis. Therefore, I mainly will apply biochemical methods like enzymatic assays, biophysical techniques like SPR, thermal shift assays, small angle X-ray scattering or ITC as well as X-ray crystallography to solve the structure of still unknown binding-sites. Fragment screening carried out by soaking approaches shall help to identify potential new inhibitors. Furthermore, I’m interested in UMPK interacting proteins that allows me to work deeper at the field of molecular biology. Dealing with all these diverse fields of science gives me the possibility to cooperate not only with colleagues of the different research groups within the Institut Pasteur but also with colleagues of the AEGIS project all over Europe.
Born and grown up in Franconia, Germany, I did my B.Sc. in Biochemistry at the University of Ulm. Afterwards I obtained my M.Sc. at the Philipps-University of Marburg where I did my thesis at the research group of Prof. Klebe at the Institute of Pharmaceutical Chemistry. Thanks to the ITN fellowship (AEGIS project) I’m now able to do my Ph.D. at the Institut Pasteur in Paris. Besides the work within an international environment at a high scientific institution it gives me also the possibility to refresh my memories in the French language and to take part at the excited Parisian lifestyle.
Astra Zeneca (3 Monate)
List of publications:
List of posters:
Identification and characterization of allosteric sites of uridine monophosphate kinase (UMPK) from Mycobacterium tuberculosis.Walter P, Munier-Lehmann H; AMR 2018 - Challenges and new concepts in antibiotics research, Paris, March 19-21, 2018
Insight into the allosteric sites of uridine monophosphate kinase (UMPK) from Mycobacterium tuberculosisWalter P, Munier-Lehmann H; How cryo Electron Microscopy meets chemical structure. Nov 14-15, 2018 Paris, (Poster)
"Structural and biophysical studies on bacterial UMP kinase as anti - TB target", AMR 2018 - Challenges and new concepts in antibiotics research, Paris, March 19-21, 2018
List of presentations:
Identification and characterisation of allosteric sites of UMP kinase.
Institut Pasteur & Sorbonne Université, Oct 25, 2019, Paris, France
Biochemistry and screening group
Department of Structural Biology and Chemistry
CNRS UMR 3528
25-28 rue du docteur Roux,